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Image Search Results
Journal: The Journal of Neuroscience
Article Title: Gap Junctions Mediate Bystander Cell Death in Developing Retina
doi: 10.1523/JNEUROSCI.23-16-06413.2003
Figure Lengend Snippet: Carbenoxolone decreased dye coupling in the scrape-loading model. Shown is control P13 rat retina scrape-loaded with RD (a) and Neurobiotin (b), which was visualized with Cy2-streptavidin. In a and b note that many Neurobiotin-labeled cells were not labeled with RD (arrowheads), indicating that they were coupled to the scrape-loaded cells (arrows). c, d, P13 retina treated with carbenoxolone (20 mg/kg, s.c., followed by 10 μm), scrape-loaded with RD and Neurobiotin. d, Neurobiotin labeling of same field as in c; only occasional cells were single labeled for Neurobiotin (arrowheads), indicating decreased coupling as compared with control (a, b). The same concentration of carbenoxolone that decreased bystander cell killing inhibited coupling to similar levels. Scale bars in a-d, 20 μm. e, The Coupling Index for retinas treated with carbenoxolone demonstrates that this gap junction inhibitor decreased the Coupling Index significantly as compared with control (asterisk indicates group significantly different from control; two-tailed t test, p < 0.01). Con, Control; Cbx, carbenoxolone.
Article Snippet: All liquid was removed, and 10 μl of 0.5% rhodamine dextran (RD; Molecular Probes, Eugene, OR) and 3%
Techniques: Labeling, Concentration Assay, Two Tailed Test